Overview
Library NameBAC library CMCMBd
Unique NameCMCMBd
OrganismCastanea mollissima (Chinese Chestnut)
Typebac_library
EcoR1 BAC library from cultivar Vanuxem.
Properties
Property NameValue
Library sourceVanuxem ramet from Greg Miller Mah series
Library source confirmationMarker genotype performed by Jeff Tomkins
Library collection commentsGreg Millers Mah series, Vanuxem, an unknown Chinese whose ramets show high blight resistance. Collected in early fall, 2006 by Greg Miller, sent to Jeff Tomkins. Five grafted ramets of one clone sent to CUGI from Greg Miller, all with the same genotype
Library availabilityPublicly available from Clemson University Genomics Institute. Order a copy of the library, filters, hybridizations, or individual clones from the Clemson University Genomics Institutes ordering site.
Library construction commentsConstructed by Chris Saski at Clemson University Genomic Institute. 384 well plant format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized EcoRI and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estimated to be 115 Kb. 92,160 clones are arrayed onto 240 plates, yielding an estimated 10X genome coverage. The library can be placed on 5 filters.