Library NameCM_MBc
Unique NameBAC Library CM_MBc
OrganismCastanea mollissima (Chinese Chestnut)
Constructed from chinese chestnut leaves, genotype Vanuxem or Mahogany, provided from Greg Miller. Constructed by Laura Georgi at Clemson University Genomic Institute. 384 well plate format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized Hind III and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estima ted to be 90 Kb. 110,592 clones are arrayed onto 288 plates, yielding an estimated 12X genome coverage. The library can be placed on 6 filters. Copies of this library, individual clones, filter sets, and other services are publicly available and may be ordered from CUGI. See the Clemson University Genomics Institutes ordering site.