Chromosome Number: 2n=30, 32 1
The following browser provides a list of biomaterials associated with this organism.
Below is a list of transcriptomes available for Liquidambar styraciflua. Click the transcriptome name for further details.
Predicted SSRs (genomic)Publication: Staton M, Best T, Khodwekar S, Owusu S, Xu T, Xu Y, Jennings T, Cronn R, Arumuganathan AK, Coggeshall M, Gailing O. Preliminary Genomic Characterization of Ten Hardwood Tree Species from Multiplexed Low Coverage Whole Genome Sequencing. PloS one. 2015 Dec 23;10(12):e0145031.
In order to develop SSR resources for ten important North American hardwood tree species, we individually barcoded and multiplex sequenced DNA from ten hardwood forest tree species using short reads produced from an Illumina HiSeq. Raw reads can be downloaded from the NCBI short read archive, project SRP021923. Bioinformatic processing included trimming, assembly, SSR identification and primer design, processing details including scripts are available. Statistics (combination of two sequenced libraries):
Confirmed Polymorphic SSRsJohn Carlson at Pennsylvania State University screened a subset of the above predicted SSRs.
Twenty four American sweetgum trees were sampled from a Pennsylvania State University provenance trial (University Park, PA). DNA was extracted using a modified CTAB method, and DNA quality was assessed on 1% agarose gels. Species primer sets were tested against all collected genotypes within a species with a PCR reaction mixture containing between 4-6 ng DNA per reaction volume, and PCR cycles as follows: 95°C-5 min, 35 cycles of 95°C- 30s, 60°C-45s, 72°C-1 min, and a final 72°C-10 min. Both amplification and polymorphism were detected on 2% agarose gels stained with 0.05% (w/v) ethidium bromide. Polymorphism was estimated based on band width.
Of the 96 tested SSRs, 44 successfully amplified and 28 were found to be polymorphic.