Summary
Name
Green Ash - Abiotic Stress Gene Expression
Resource Type
Gene Expression Profile
Organism
Publication
Lane T, Best T, Zembower N, Davitt J, Henry N, Xu Y, Koch J, Liang H, McGraw J, Schuster S, Shim D. The green ash transcriptome and identification of genes responding to abiotic and biotic stresses. BMC genomics. 2016 Dec;17(1):702.
Description
This project aims to elucidate the molecular response of hardwood tree seedlings to varying abiotic stresses. Stress experiments include ozone exposure, mechanical damage (wounding), heat, drought, and cold. A number of different adult tissue types were also profiled. Expression profiles were determined by mapping reads to the set of assembled transcripts. Reads were mapped with Bowtie2 and counted using HTSeq. Values were normalized using RPKM (Reads Per Kilobase Million) for gene expression profiles.
Expression Data

This analysis contains expression data for 107611 features across 55 biosamples.

Expression Data
Program, Pipeline, Workflow or Method Name
HTSeq; RPKM
Program Version
n/a
Date Performed
Thursday, July 17, 2014 - 21:00
Data Source
Source Name
: Fraxinus pennsylvanica 120313
Source Version
: 120313
Gene Annotations
Biosamples
NameTissueTreatmentDescription
24-GA-CLleafcold(4C)-24hrGreen ash-open pollinated offspring of Green Ash Mother (6 pooled seedlings)
24-GA-CPpetiolecold(4C)-24hrGreen ash-open pollinated offspring of Green Ash Mother (6 pooled seedlings)
24-GA-CRrootcold(4C)-24hrGreen ash-open pollinated offspring of Green Ash Mother (6 pooled seedlings)
FS-1phloem and barknoneFS Tree 1, summer sample of phloem and bark
FS-10-eggphloem and barkafter EAB damageFS Tree 10, fall sample of phloem and bark after EAB feeding
FS-11phloem and barknoneFS Tree 11, summer sample of phloem and bark
FS-12-eggphloem and barkEAB feedingFS Tree 12, fall sample of phloem and bark after EAB feeding
FS-2-eggphloem and barkafter EAB damageFS Tree 2, fall sample of phloem and bark after EAB feeding
FS-3phloem and barknoneFS Tree 3, summer sample of phloem and bark
FS-4-eggphloem and barkafter EAB damageFS Tree 4, fall sample of phloem and bark after EAB feeding

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