Summary
Name
Black Walnut - Abiotic Stress Gene Expression
Resource Type
Gene Expression Profile
Organism
Program, Pipeline, Workflow or Method Name
HTSeq; RPKM
Program Version
n/a
Date Performed
Tuesday, July 8, 2014 - 21:00
Data Source
Source Name
: Juglans nigra 120313
Source Version
: 120313
Description and Download
Description: 

This project aims to elucidate the molecular response of hardwood tree seedlings to varying abiotic stresses. Experiments include ozone exposure, mechanical damage as well as a number of different adult tissue types.

Expression profiles were determined by mapping reads to the set of assembled transcripts. Reads were mapped with Bowtie2 and counted using HTSeq. Values were normalized using RPKM (Reads Per Kilobase Million) for gene expression profiles.

Expression Data Files

Black Walnut Raw Reads Counts

Biosamples
NameTissueTreatmentDescription
BW-M-20undamaged leavesnoneBlack Walnut-Sparrow-Mother tree
BW-M-22undamaged twigsnoneBlack Walnut-Sparrow-Mother tree
BW-D-23damaged twigsmechanical woundingBlack Walnut-Schlesser-Father tree
BW-D-22undamaged twigsnoneBlack Walnut-Schlesser-Father tree
BW-M-23damaged twigsmechanical woundingBlack Walnut-Sparrow-Mother tree
BW-OW-9leafcontrol chamber ozone 10ppb+ mecanical wounding on day 28Black Walnut-offspring of Sparrow and Schlesser two yr.old (6 pooled seedlings)
BW-OW-11leafozone exposure 125ppb for 29 day + mecanical wounding on day 28Black Walnut-offspring of Sparrow and Schlesser two yr.old (6 pooled seedlings)
BW-M-19dormant twigsnoneBlack Walnut-Sparrow-Mother tree
BW-OW-12leafozone exposure 225ppb for 29 day + mecanical wounding on day 28Black Walnut-offspring of Sparrow and Schlesser two yr.old (6 pooled seedlings)
BW-D-28catkins (male flowers- very mature)noneBlack Walnut-Schlesser-Father tree

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