A “PseudoSanger”-like approach was used to assemble two paired-end read libraries which were assembled using Newbler 2.9. After processing the final assembly consisted of 3887 scaffolds with a total length of 550.5 Mb and an N50 of 623.8 kb with L50 of 240 scaffolds. Note, 1206 unanchored scaffolds were concatenated to form a composite entity known as ‘Chr30’ that was submitted as individual (non-concatenated) scaffolds in NCBI Genbank. Genome accuracy was evaluated by comparing the assembled contigs for 22 clones from a BAC library of A. chinensis Red Female 1. This resulted from using a different technology and different assembly path (Newbler). BUSCO analysis was used to evaluate genome completeness for comparison of the published chromosomal sequences for ‘Hongyang'. Red5 contained 1364 (94.7%) ‘complete’ BUSCOs and in comparison, ‘Hongyang’ contained 1358 (94.3%) ‘complete’ BUSCOs. Of the 47,384 A. chinensis EST sequences that were mapped to the Red5 chromosomes, only 580 had no homology. In comparison, when the ESTs were aligned to the published chromosomes of ‘Hongyang’, 3295 had no homology to any region, suggesting Red5 and Hongyang have a comparable gene space assembly.
Downloads:
|
All Gene Models CDS (fasta format)
|
|
|
All Gene Models Protein (fasta format)
|
|
|
Genome Scaffolds (fasta format)
|
|
|
GFF3
|
|
Source:
Pilkington, S. M., Crowhurst, R., Hilario, E., Nardozza, S., Fraser, L., Peng, Y., … Schaffer, R. J. (2018). A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants. BMC Genomics, 19, 257.
Source
