Resource Type
Transcriptome Assembly
de novo Dogwood (Cornus florida)
Program Version
Newbler (Roche) [30-Nov-2016]
Date Performed
Monday, December 5, 2016 - 20:00
Data Source
Source Name
: de novo assembly
Program, Pipeline, Workflow or Method Name
Newbler (Roche)
Number of contigs
Average Contig Length
Description and Download

Assembly Statistics

Number of Transcripts 43,158
Transcript N50 1,206 bp
Transcript Average Length 1,086 bp
Number of Proteins 39,577
Protein N50 265 aa
Protein Average Length 231 aa


Assembled sequence data:

Putative Transcripts (fasta format)

Predicted ORFs (fasta format)

Functional Annotation

BLASTx analysis against Swiss-prot protein database with an e-value cut-off of 1e-5.

File (tsv file)

BLASTx analysis against Trembl protein database, only plant entries with an e-value cut-off of 1e-5.

File (tsv file)

InterProScan searches with GO term annotation:

File (tsv file)

SSR Pipeline

Excel file with statistics, SSR motifs and primers (? high quality markers)

Fasta file of transcripts with SSR motifs

Read Statistics

Two cultivars of C. florida were used for sequencing: ‘Appalachian Spring’ and ‘Cherokee Brave’. RNA was extracted from leaf, fully expanded bract, and flower tissue. RNA was combined and sequenced to yield 580Mbp of sequence data from 1,621,644 reads.